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ARTICLE |

THE CONCENTRATION OF ANTIPNEUMOCOCCUS SERUM

LLOYD D. FELTON, M.D.
JAMA. 1930;94(24):1893-1896. doi:10.1001/jama.1930.02710500011005.
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An important consideration in the study of antipneumococcus serum is a satisfactory valuation of its therapeutic activity. This immune serum, in contradistinction to the antitoxic serums, is classified with the antibacterial serums, and as such gives ample demonstration of containing all the known varieties of antibodies. However, although not entirely satisfactory in determining its therapeutic activity, the present method of choice is the estimation of the so-called protective antibody by means of some form of mouse protection test. For this test two procedures are advocated: first, the injection of a constant dose of serum with a varying number of organisms, the standard method of the U. S. Hygienic Laboratory1 based on work of Neufeld1a and that done at the Rockefeller Institute,2 and, second, the injection of a constant dose of organism with varying dilutions of serum.3 These two methods in my hands have given roughly the

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