We are dealing in this paper only with resistant organisms, which normally occur in commercial, unsterilized ligatures, as they are able to survive the drastic cleaning, bleaching and drying processes through which the raw sheep gut must pass before it becomes a finished ligature or suture.
In the course of the experimentation to determine the temperature necessary to insure sterility of the product, more than a hundred cultures were obtained, both from the raw, unsterilized ligatures and from those heated at various temperatures. These cultures were examined, and from them fourteen typical specimens were selected which included all the varieties observed. From these fourteen cultures five distinct types of bacteria were isolated: three spore-forming bacilli and two cocci. All were aerobic and facultatively anaerobic and grew at both 20 and 37 C., though somewhat more quickly at the latter temperature.
A careful search of the literature failed to disclose any