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ARTICLE |

STAINING OF BACTERIA IN TISSUES FOR BACTERIOLOGIC STUDY OF PNEUMONIC FOCI:  A MODIFICATION OF METHOD, AS APPLIED ESPECIALLY TO THE PFEIFFER BACILLUS

Kuyoshi Sato, M.D.
JAMA. 1919;72(19):1365. doi:10.1001/jama.1919.26110190004009e.
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ABSTRACT

There are many rules and directions laid down for the staining of bacteria in tissue, especially when microphages and macrophages are concerned; but the most important requirement is that, while the cell protoplasm shall be moderately bleached, the nuclei and the bacteria shall become and remain plainly visible. Last fall a pandemic of influenza swept over Japan. In investigations undertaken to ascertain the morphology and the incidence of the bacterial phenomena, many of the staining methods did not produce the best results, but with my modification of the carbolfuchsin staining-fluid satisfactory results were achieved.

MODIFIED STAINING METHOD 

  1. The frozen sections should be cut as thin as possible and glued to the object glass by means of an egg-white glycerin mixture.

  2. They should be stained for a period of from ten to twenty minutes in a solution of carbolfuchsin which has been prepared by dissolving 1 c.c. of carbolfuchsin in 10

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