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February 8, 1919, Vol 72, No. 6 >
ARTICLE | February 8, 1919

PREPARATION OF MEDIUMS: A NEW HYDROGEN-ION CONCENTRATION METHOD

Lyman J. Strong, M.D.
JAMA. 1919;72(6):413. doi:10.1001/jama.1919.26110060003009c.
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ABSTRACT

That bacteria may grow, the invironment must be of about the same acidity or alkalinity; for bacteria to be pathogenic, this environment must be near the alkalinity of the human tissues. The adjustment of mediums to this alkalinity by methods now in common use is time consuming and often unsatisfactory. Carefully made standard solutions, with fine titration, often produce a product after sterilization that is found to have an entirely different reaction. If separate tubes or flasks are investigated, one is surprised to find the wide variation that exists in different containers of apparently the same medium. This is especially true of sugar mediums.

Much work has been done on indicators, but each has its fault as a general indicator: Litmus is not sensitive enough. Andrade's is suited only for aldehyd formers. Fuchsin does not have a suitable end-point. Brilliant green is inhibitory. Phenolphthalein is not indicating in acid. Others

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