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Ivan C. Hall, M.S.
JAMA. 1919;72(4):274-275. doi:10.1001/jama.1919.26110040001016.
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Repeated plating and streaking out on agar plates are universally acknowledged to be fairly reliable procedures for the isolation of aerobic bacteria irrespective of the possible concurrence of obligative anaerobes, for the latter, if present, are completely eliminated by the conditions of aerobic culture. But the same procedures applied under anaerobic conditions for the isolation of obligative anaerobes from cultures mixed with aerobes involve much uncertainty owing to the fact that many aerobes are also facultative anaerobes. It is therefore necessary to eliminate all aerobes from the culture before attempting the purification of obligative anaerobes by any method of surface colony isolation. It is an easy matter to eliminate nonsporulating contaminations by heating cultures containing sporulating anaerobes to a temperature sufficient to destroy vegetative forms alone; but selective heating is a total failure for the elimination of hay bacilli, since most of these organisms are less susceptible to heat than


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