Although there are many methods for making anaerobic plate cultures that give good results, there are few described for partial-tension plates. Wherry and Oliver1 have devised a technic which they thus describe:
In making partial-tension plates we have inverted the inoculated plate upon a glass plate on which is fastened (with a small piece of plasticine) a smaller open Petri dish containing a freshly inoculated culture of B. subtilis. The inverted dish should be high enough to avoid any contact with the smaller dish. The edges of the inverted dish are then sealed with plasticine as recommended by Lentz2 for the growth of anaerobes. Plates made in this way and sealed to pieces of window glass of suitable size may be stacked in the incubator.
In the use of this method as a routine in an investigation of the adaptation of certain organisms to various oxygen tensions, a