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JAMA. 1910;55(6):517-519. doi:10.1001/jama.1910.04330060069027.
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Qualitative examination having indicated the presence of alcohol, hydrogen ions, sulphate ions and traces of iron, phosphates, sodium and potassium, the following quantitative estimations were made: Alcohol: Alcohol was estimated according to the method of the Bureau of Chemistry, Department of Agriculture, Bulletin No. 107 (Revised), 1908, p. 83. The results of the estimations indicated the presence of 44.10 per cent. alcohol. Sulphuric acid: The acidity of the preparation was determined by direct titration with tenth-normal potassium hydroxid solution using methyl orange as an indicator. The following results were obtained: (a) 10 c.c. of a 1 to 10 dilution of Lung Germine with water required 7.91 c.c. tenth-normal potassium hydroxid and (b) required 7.99 c.c. of the alkali; an average of 7.95 c.c.. This is equivalent to 0.0385 gm. sulphuric acid (H2SO4). As a check the sulphate (SO4) content was estimated in the finished titrations by precipitation with barium chlorid and weighing as barium sulphate. The following results were obtained: (a) yielded 0.0902 gm. barium sulphate and (b) yielded 0.0905 gm. barium sulphate; average 0.09035 gm. equivalent to 0.0380 gm. sulphuric acid (H2SO4). This agrees sufficiently with the result obtained by titration, i. e., 0.0385 gm., to demonstrate that the latter is correct. The sulphuric acid found by the above method is equivalent to 3.98 per cent. by weight. Ash: The ash was determined in the usual way by evaporation of the preparation and ignition of the residue. 10 c.c. of Lung Germine yielded (a) 0.0039 and (b) 0.0041 gm. ash, an average of 0.0040 gm. or 0.041 per cent.


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