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Human Genome Studies Expected to Revolutionize Cancer Classification

JAMA. 1999;282(10):927-928. doi:10.1001/jama.282.10.927.
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Figures

Grahic Jump LocationImage not available.

Microarrays allow scientists to compare gene expression in normal and tumor cells. A robot places tiny droplets containing gene fragments on a glass slide, creating a panel for the analysis of gene activity. Genetic material from normal and tumor tissues is extracted and transcribed into complementary DNA (cDNA), which is then labeled with fluorescent dyes. If a cDNA "probe" binds to a gene fragment on the slide, this indicates that the gene in the tissue specimen is active. A specialized scanning microscope measures the intensity of each fluorescent spot, which is an indicator of the degree to which a specific gene is expressed in a particular tissue. (Credit: National Human Genome Research Institute)
Progression of loss of 18F-dopa storage in an initially asymptomatic identical twin of a patient with PD who 5 years later became clinically affected. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a normal subject, a relative who was asymptomatic but subclinically affected by PD, and a patient clinically affected by PD. (Credit: David Brooks, MD)
Basal ganglia and thalamic retention of PK11195, a marker of activated microglial cells, in a patient with PD indicates the presence of an active inflammatory response to the disease. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a patient with PD at baseline and again 2 years later shows clear loss of dopamine storage along with clinical progression on the Unified Parkinson's Disease Rating Scale. (Credit: David Brooks, MD)

Grahic Jump LocationImage not available.

Microarrays allow scientists to compare gene expression in normal and tumor cells. A robot places tiny droplets containing gene fragments on a glass slide, creating a panel for the analysis of gene activity. Genetic material from normal and tumor tissues is extracted and transcribed into complementary DNA (cDNA), which is then labeled with fluorescent dyes. If a cDNA "probe" binds to a gene fragment on the slide, this indicates that the gene in the tissue specimen is active. A specialized scanning microscope measures the intensity of each fluorescent spot, which is an indicator of the degree to which a specific gene is expressed in a particular tissue. (Credit: National Human Genome Research Institute)
Progression of loss of 18F-dopa storage in an initially asymptomatic identical twin of a patient with PD who 5 years later became clinically affected. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a normal subject, a relative who was asymptomatic but subclinically affected by PD, and a patient clinically affected by PD. (Credit: David Brooks, MD)
Basal ganglia and thalamic retention of PK11195, a marker of activated microglial cells, in a patient with PD indicates the presence of an active inflammatory response to the disease. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a patient with PD at baseline and again 2 years later shows clear loss of dopamine storage along with clinical progression on the Unified Parkinson's Disease Rating Scale. (Credit: David Brooks, MD)

Grahic Jump LocationImage not available.

Microarrays allow scientists to compare gene expression in normal and tumor cells. A robot places tiny droplets containing gene fragments on a glass slide, creating a panel for the analysis of gene activity. Genetic material from normal and tumor tissues is extracted and transcribed into complementary DNA (cDNA), which is then labeled with fluorescent dyes. If a cDNA "probe" binds to a gene fragment on the slide, this indicates that the gene in the tissue specimen is active. A specialized scanning microscope measures the intensity of each fluorescent spot, which is an indicator of the degree to which a specific gene is expressed in a particular tissue. (Credit: National Human Genome Research Institute)
Progression of loss of 18F-dopa storage in an initially asymptomatic identical twin of a patient with PD who 5 years later became clinically affected. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a normal subject, a relative who was asymptomatic but subclinically affected by PD, and a patient clinically affected by PD. (Credit: David Brooks, MD)
Basal ganglia and thalamic retention of PK11195, a marker of activated microglial cells, in a patient with PD indicates the presence of an active inflammatory response to the disease. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a patient with PD at baseline and again 2 years later shows clear loss of dopamine storage along with clinical progression on the Unified Parkinson's Disease Rating Scale. (Credit: David Brooks, MD)

Grahic Jump LocationImage not available.

Microarrays allow scientists to compare gene expression in normal and tumor cells. A robot places tiny droplets containing gene fragments on a glass slide, creating a panel for the analysis of gene activity. Genetic material from normal and tumor tissues is extracted and transcribed into complementary DNA (cDNA), which is then labeled with fluorescent dyes. If a cDNA "probe" binds to a gene fragment on the slide, this indicates that the gene in the tissue specimen is active. A specialized scanning microscope measures the intensity of each fluorescent spot, which is an indicator of the degree to which a specific gene is expressed in a particular tissue. (Credit: National Human Genome Research Institute)
Progression of loss of 18F-dopa storage in an initially asymptomatic identical twin of a patient with PD who 5 years later became clinically affected. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a normal subject, a relative who was asymptomatic but subclinically affected by PD, and a patient clinically affected by PD. (Credit: David Brooks, MD)
Basal ganglia and thalamic retention of PK11195, a marker of activated microglial cells, in a patient with PD indicates the presence of an active inflammatory response to the disease. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a patient with PD at baseline and again 2 years later shows clear loss of dopamine storage along with clinical progression on the Unified Parkinson's Disease Rating Scale. (Credit: David Brooks, MD)

Grahic Jump LocationImage not available.

Microarrays allow scientists to compare gene expression in normal and tumor cells. A robot places tiny droplets containing gene fragments on a glass slide, creating a panel for the analysis of gene activity. Genetic material from normal and tumor tissues is extracted and transcribed into complementary DNA (cDNA), which is then labeled with fluorescent dyes. If a cDNA "probe" binds to a gene fragment on the slide, this indicates that the gene in the tissue specimen is active. A specialized scanning microscope measures the intensity of each fluorescent spot, which is an indicator of the degree to which a specific gene is expressed in a particular tissue. (Credit: National Human Genome Research Institute)
Progression of loss of 18F-dopa storage in an initially asymptomatic identical twin of a patient with PD who 5 years later became clinically affected. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a normal subject, a relative who was asymptomatic but subclinically affected by PD, and a patient clinically affected by PD. (Credit: David Brooks, MD)
Basal ganglia and thalamic retention of PK11195, a marker of activated microglial cells, in a patient with PD indicates the presence of an active inflammatory response to the disease. (Credit: David Brooks, MD)
Striatal 18F-dopa uptake in a patient with PD at baseline and again 2 years later shows clear loss of dopamine storage along with clinical progression on the Unified Parkinson's Disease Rating Scale. (Credit: David Brooks, MD)

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