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Esther R. Ziegler, M.S.; Arnold E. Osterberg, Ph.D.; Mildred Hovig, R.N.
JAMA. 1940;114(14):1341-1342. doi:10.1001/jama.1940.62810140001012.
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The use of refrigerated blood is of intense interest to most physicians and at the present time a scheme for its management has been worked out. From the beginning of the use of such blood at the Mayo Clinic the amount of blood in the refrigerator at any one time has been minimized to a small enough quantity so that each day or two fresh blood will be collected and placed in the refrigerator. It is only occasionally that blood which has been in the icebox for many days is used. The desire to study the quality of older blood and relatively fresh blood has led us to investigate several properties of blood and how they are altered by short chilling and prolonged chilling. The blood is citrated and kept in the refrigerator at a temperature of 34 F. in a 500 cc. container used for intravenous administration. An interesting


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