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JAMA. 1935;105(5):372-373. doi:10.1001/jama.1935.02760310046016.
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SIGNIFICANCE OF CALCIUM ION CONCENTRATIONS IN THE BLOOD  At least part of the diffusible calcium in the blood serum is in the form of free ionic calcium. There is reason to believe that this form is of primary physiologic importance. Two schools of thought, both supported by experimental evidence, have developed with regard to the state of this diffusible calcium. One has held that all or nearly all the calcium not bound to protein is ionized. The other believes that, of each 5 mg. of calcium not bound to protein, not more than 2 mg. can be in the ionized state. In order to account for the remaining 3 mg. a diffusible form of calcium, bound to some citrate-like substance, as yet unidentified, has been postulated. In view of the uncertainty as to the conditions present in the fluids of the normal body, it is not surprising that interpretation of

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