The sequencing of the human genome, the ability to perform genome-wide association studies, and the development of high-throughput nucleotide sequencing support the goals of personalized medicine. “Next generation” sequencing platforms now provide much higher resolution of DNA than was possible with microarray-based–single nucleotide polymorphism (SNP) detection. Other breakthrough technologies such as the ability to sequence DNA from single cells, targeted mRNA molecules, and methylated regions of DNA; DNA fragment length analysis; isolation of circulating tumor cells; and the ability to use paraffin-embedded tissue for mRNA sequencing will make genotyping faster, easier, less expensive, better, and ultimately more readily available.
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