In Reply: In response to Drs O'Regan and Fleming,
our conclusion that osteopontin is up-regulated in ovarian cancer was based
upon RNA expression in pure cultures of human ovarian cancer epithelial cells
and pure healthy human surface epithelium. Thus, our results cannot be attributed
to macrophage production. However, we agree it is possible that additional
isoforms of osteopontin may have greater or lesser specificity for ovarian
cancer, and should be investigated further.
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