To the Editor: Amoebic encephalitis is a potentially lethal infection caused by several genera of amoeba, including Naegleria fowleri, Acanthamoeba species, Balamuthia mandrillaris, or Entamoeba histolytica.1- 3 We report a case of amoebic encephalitis caused by Sappinia diploidea, a species from a genus of free-living amoeba not previously known to be pathogenic to humans.
The characteristic double nucleus of S diploidea is evident in 3 trophozoites in the brain (white arrowheads) (hematoxylin-eosin).
Trophozoites of S diploidea surround a blood vessel in the brain. Cytoplasm is stained bright blue with Giemsa and shows the characteristic large vacuole (black arrowheads).
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